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UID:20260805T170339EDT-4897g1cPFv@132.216.98.100
DTSTAMP:20260805T210339Z
DESCRIPTION:Robert E. Campbell\nAssistant Professor\nUniversity of Alberta
 \n \nAt the heart of the Campbell group's research program lies the\nextra
 ordinarily useful family of fluorescent proteins (FPs) which\nincludes the
  green FP (GFP) from Aequorea jellyfish and\nits numerous homologues of va
 rious color from coral. All FPs share\nthe ability to autonomously generat
 e a visible wavelength\nfluorophore from a sequence of 3 amino acids locat
 ed in the center\nof their barrel-like structures. FPs are credited with s
 parking the\nexplosive and continuing growth in the popularity of live cel
 l\nfluorescence microscopy\; culminating in the 2008 Nobel Prize in\nChemi
 stry being awarded to 3 pioneers in this area\; Shimomura\,\nChalfie\, and
  Tsien.\nSince its inception in 2003\, the Campbell research group has\nwo
 rked to mould (that is\, engineer) FPs into improved tools for\naddressing
  fundamental questions in life science. For example\, we\nhave developed s
 everal improved alternatives to some of the\ntraditionally preferred hues 
 of FPs [1\,2\,3] and exploited their\nimproved properties in new applicati
 ons. Some of our novel\napplications include the use of FPs for investigat
 ing peptide\nstructure in vivo [4]\, dual-FRET imaging of caspase activati
 on\nduring apoptosis [5]\, and a FP-based assay of nucleoside\ntransporter
  activity (with Joe Casey) [6]. In this seminar I will\ndiscuss some of ou
 r most recent efforts to create FP variants that\nhave physical or spectra
 l properties that are either improved\nrelative to existing variants or ar
 e completely novel and have not\nbeen previously reported. As the title of
  this seminar indicates\,\nthis discussion will focus on 3 aspects of FPs\
 ; chain topology\,\nsurface properties\, and chromophore chemistry. Specif
 ically\, I will\npresent our most recent progress in the following areas: 
 creation\nof circularly permuted red FPs through manipulation of topology\
 ;\nengineering of monomeric and heterodimeric FPs through manipulation\nof
  surface properties\; and engineering of photostable and\nphotconvertable 
 FPs by manipulation of chromophore chemistry and\nenvironment.\n
DTSTART:20090917T200000Z
DTEND:20090917T200000Z
LOCATION:McIntyre Medical Building\, CA\, QC\, Montreal\, H3G 1Y6\, 3655 pr
 omenade Sir William Osler
SUMMARY:Robert E. Campbell 'Engineering fluorescent proteins into new bioto
 ols: manipulation of protein surfaces\, topology\, and chromophores'
URL:/channels/event/robert-e-campbell-engineering-fluo
 rescent-proteins-new-biotools-manipulation-protein-surfaces-topolo-108899
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