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UID:20250805T212906EDT-1624bsh3on@132.216.98.100
DTSTAMP:20250806T012906Z
DESCRIPTION:Applied Mass Spectrometry Workshops. This talk is opened to any
 one interested in learning more about Mass Spectrometry and its applicatio
 ns will obtain a great deal from these monthly workshops.\n\nGuest speaker
 : Dr. Christopher Thibodeaux\, Department of Chemistry\, ɬÀï·¬
 .\n\nThis lecture will focus on the application of mass spectrometry to st
 udy the involvement of protein conformational changes in biosynthetic enzy
 me function. Specifically\, I will discuss the use of hydrogen/deuterium e
 xchange mass spectrometry (HDX-MS) and native mass spectrometry coupled to
  ion mobility separations. HDX-MS measures how perturbations (substrate bi
 nding\, mutagenesis\, etc.) alter the exchange rate of solvent deuterium i
 nto the backbone amide moiety of proteins. This exchange rate is largely a
  function of protein secondary structure and\, thus\, provides information
  on the location of substrate binding sites and other perturbation-induced
  structural changes in the protein. In native mass spectrometry\, proteins
  (or non-covalently bound protein-substrate complexes) are transferred fro
 m solution into the gas phase in a natively folded state using nano-electr
 ospray ionization. Once in the gas phase\, the conformational distribution
  of the folded protein can be probed by ion mobility separations. The para
 meters derived from these measurements can then be correlated to structura
 l parameters derived from other techniques in order to build mechanistic m
 odels for the involvement of protein conformational changes in enzyme func
 tion. Finally\, I will discuss how my lab is applying HDX-MS and native MS
  approaches to study the multifunctional enzymes involved in peptide natur
 al product biosynthesis.\n\nABOUT THE SPEAKER\n\nChristopher J. Thibodeaux
  is a native of Louisiana\, where he graduated valedictorian with bachelor
 's degrees in Biochemistry\, Botany\, and Chemistry from Louisiana State U
 niversity. He then entered graduate school in the lab of Hung-wen Liu at t
 he University of Texas\, Austin\, where his Ph.D. focused on elucidating t
 he chemical and kinetic mechanisms of enzyme catalysis. Following graduati
 on\, he had postdoctoral stints in the labs of Taekjip Ha and Wilfred van 
 der Donk at the University of Illinois\, Urbana-Champaign\, where he studi
 ed biomolecular single molecule fluorescence spectroscopy and peptide natu
 ral product biosynthesis\, respectively. In 2016\, he began his independen
 t career in the Chemistry Department at ɬÀï·¬\, where he is cu
 rrently an assistant professor. His research is broadly aimed at combattin
 g the problem of antimicrobial resistance by understanding the detailed mo
 lecular mechanisms of enzymes that synthesize structurally complex antimic
 robial compounds\, discovering novel antimicrobial compounds by genome min
 ing\, and by investigating the mechanisms used by bacteria to establish bi
 ofilms.\n
DTSTART:20190121T170000Z
DTEND:20190121T180000Z
LOCATION:MS2-022\, Macdonald-Stewart Building\, CA\, QC\, St Anne de Bellev
 ue\, H9X 3V9\, 21111 Lakeshore Road
SUMMARY:Using Hydrogen/Deuterium exchange and native mass spectrometry to s
 tudy peptide antibiotics biosynthesis
URL:/macdonald/channels/event/using-hydrogendeuterium-
 exchange-and-native-mass-spectrometry-study-peptide-antibiotics-biosynthes
 is-293296
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