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DTSTAMP:20250506T160613Z
DESCRIPTION:'Deconstructing biology with simple single-molecule imaging: Co
 ntrolling conformation\, confinement\, and concentration'\n\nSabrina Lesli
 e (ɬÀï·¬)\n	Tuesday January 15\, 12-1pm\n	Montreal Neurological 
 Institute\, DeGrandpre Communications Centre\n	\n	Abstract: Molecular biolog
 y is messy and complex. The future of life sciences research\, drug develo
 pment\, and many other fields depends on our ability to unravel the comple
 x\, molecular phenomena that underlie cellular function. Using currently a
 vailable technologies\, it is still challenging and costly to conduct sing
 le-molecule measurements that can potentially reveal the true complexity o
 f life at the molecular scale. Convex Lens-induced Confinement (CLiC) micr
 oscopy is a simple\, general method of imaging molecular interactions one 
 molecule at a time. By mechanically confining molecules to the field of vi
 ew\, CLiC eliminates the complexity and potential biases inherent in metho
 ds such as optical tweezers and TIRF. In this talk I discuss how we employ
  CLiC to investigate how DNA supercoiling regulates the dynamic unwinding 
 of specific target sites\, and the activity of molecules binding to these 
 sites – important biophysical questions for medicine and antisense oligonu
 cleotide drug development (Scott et. al.\, Nucleic Acids Research 2018). I
  will also introduce new and emerging areas of exploration with CLiC inclu
 ding protein droplets\, nanoparticle dynamics\, CRISPR-Cas9 search process
 es\, and therapeutics applications.\n
DTSTART:20190115T170000Z
DTEND:20190115T180000Z
LOCATION:deGrandpre Communications Centre\, Montreal Neurological Institute
 \, CA\, QC\, Montreal\, H3A 2B4\, 3801 rue University
SUMMARY:Seminar Series in Quantitative Life Sciences and Medicine
URL:/qls/channels/event/seminar-series-quantitative-li
 fe-sciences-and-medicine-292955
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